MMACHC Mouse Monoclonal Antibody [Clone ID: OTI1A4]

CAT#: TA504954

MMACHC mouse monoclonal antibody, clone OTI1A4 (formerly 1A4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC. Positive lysates LY402444 (100ug) and LC402444 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MMACHC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504954)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MMACHC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504954)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MMACHC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504954)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MMACHC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504954)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MMACHC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504954)

• Anti-MMACHC mouse monoclonal antibody (TA504954) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMACHC (RC223846).

• HEK293T cells transfected with either RC223846 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MMACHC antibody (TA504954), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1A4
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MMACHC(NP_056321) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.5 kDa
• Gene Name: metabolism of cobalamin associated C
• Database Link:
  NP_056321
  Entrez Gene 25974 Human
  UniProt ID Q9Y4U1
• Background: The exact function of the protein encoded by this gene is not known, however, its C-terminal region shows similarity to TonB, a bacterial protein involved in energy transduction for cobalamin (vitamin B12) uptake. Hence, it is postulated that this protein may have a role in the binding and intracellular trafficking of cobalamin. Mutations in this gene are associated with methylmalonic aciduria and homocystinuria type cblC. [provided by RefSeq]
• Synonyms: cblC