RFC2 Mouse Monoclonal Antibody [Clone ID: OTI4E1]

CAT#: TA504956

RFC2 mouse monoclonal antibody, clone OTI4E1 (formerly 4E1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2 (RC201138, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC2. Positive lysates LY419017 (100ug) and LC419017 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RFC2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  HEK293T cells transfected with either RC201138 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RFC2 antibody (TA504956), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4E1
• Applications: FC, WB
• Recommended Dilution: WB 1:200~2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protei fragment corresponding to amino acids 1-234 of human RFC2(NP_002905) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.1 kDa
• Gene Name: replication factor C subunit 2
• Database Link:
  NP_002905
  Entrez Gene 19718 MouseEntrez Gene 116468 RatEntrez Gene 5982 Human
  UniProt ID P35250
• Background: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
• Synonyms: RFC40

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: DNA replication, Mismatch repair, Nucleotide excision repair