STING (TMEM173) Mouse Monoclonal Antibody [Clone ID: OTI4H8]

CAT#: TA505024

TMEM173 mouse monoclonal antibody, clone OTI4H8 (formerly 4H8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEM173 (RC208418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEM173. Positive lysates LY405006 (100ug) and LC405006 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and TMEM173-Knockout HeLa cells (KO, Cat# LC835106) were separated by SDS-PAGE and immunoblotted with anti-TMEM173 monoclonal antibody TA505024 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TMEM173 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505024)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TMEM173 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505024)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TMEM173 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505024)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TMEM173 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505024)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TMEM173 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505024)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TMEM173 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505024)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TMEM173 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505024)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TMEM173 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505024)

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Specifications

Product Data

• Clone Name: OTI4H8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TMEM173(NP_938023) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42 kDa
• Gene Name: transmembrane protein 173
• Database Link:
  NP_938023
  Entrez Gene 340061 Human
  UniProt ID Q86WV6
• Synonyms: ERIS; hMITA; hSTING; MITA; MPYS; NET23; SAVI; STING

Reference Data

• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway