PLPBP Mouse Monoclonal Antibody [Clone ID: OTI1G2]

CAT#: TA505162

PROSC mouse monoclonal antibody, clone OTI1G2 (formerly 1G2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PROSC (Cat# RC200853, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROSC(Cat# TA505162). Positive lysates LY416132 (100ug) and LC416132 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PROSC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PROSC mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PROSC mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PROSC mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Anti-PROSC mouse monoclonal antibody (TA505162) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PROSC (RC200853).

• HEK293T cells transfected with either RC200853 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PROSC antibody (TA505162), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1G2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:200~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PROSC(NP_009129) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.2 kDa
• Gene Name: pyridoxal phosphate binding protein
• Database Link:
  NP_009129
  Entrez Gene 11212 Human
  UniProt ID O94903
• Synonyms: FLJ11861