OTUB1 Mouse Monoclonal Antibody [Clone ID: OTI5F6]

CAT#: TA505198S

OTUB1 mouse monoclonal antibody, clone OTI5F6 (formerly 5F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB1 (RC210648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB1. Positive lysates LY402607 (100ug) and LC402607 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and OTUB1-Knockout 293T cells (KO, Cat# LC810883) were separated by SDS-PAGE and immunoblotted with anti-OTUB1 monoclonal antibody TA505198, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Anti-OTUB1 mouse monoclonal antibody (TA505198) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OTUB1 (RC210648).

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  HEK293T cells transfected with either RC210648 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-OTUB1 antibody (TA505198), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI5F6
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant rotein of human OTUB1(NP_060140) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.1 kDa
• Gene Name: OTU deubiquitinase, ubiquitin aldehyde binding 1
• Database Link:
  NP_060140
  Entrez Gene 293705 RatEntrez Gene 55611 Human
  UniProt ID Q96FW1
• Background: The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: HSPC263; OTB1; OTU1

Reference Data

• Protein Families: Protease