PCDH7 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2G6]

CAT#: TA505452BM

PCDH7 mouse monoclonal antibody,clone 2G6, HRP conjugated

Product Images

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human PCDH7 plasmid (RC213200, hPCDH7 va), human PCDH7 plasmid (RC223033, hPCDH7 vc), human PCDH7 plasmid (RC230683, hPCDH7 vd), mouse PCDH7 plasmid (MR214196, mPCDH7 v1), mouse PCDH7 plasmid (MR214198, mPCDH7 v2) using anti-PCDH7 antibody TA505452 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-PCDH7 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

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  Western blot analysis of extracts (50ug per lane) from 3 cell lines lysates by using anti-PCDH7 monoclonal antibody(TA505452, 1:500)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PCDH7 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Anti-PCDH7 mouse monoclonal antibody (TA505452) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PCDH7 (RC223033).

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Specifications

Product Data

• Clone Name: OTI2G6
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PCDH7(NP_115833) produced in HKE293T cell.
• Formulation: PBS (pH 7.4) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 132.4 kDa
• Gene Name: protocadherin 7
• Database Link:
  NP_115833
  Entrez Gene 54216 MouseEntrez Gene 5099 Human
  UniProt ID O60245
• Background: This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The gene encodes a protein with an extracellular domain containing 7 cadherin repeats. The gene product is an integral membrane protein that is thought to function in cell-cell recognition and adhesion. Alternative splicing yields isoforms with unique cytoplasmic tails. [provided by RefSeq, Jul 2008]
• Synonyms: BH-Pcdh; BHPCDH; PPP1R120

Reference Data

• Protein Families: Druggable Genome, Transmembrane