CD272 (BTLA) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4D6]

CAT#: TA505536BM

BTLA mouse monoclonal antibody,clone 4D6, HRP conjugated

Product Images

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505536)

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA (RC219458, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTLA. Positive lysates LY403628 (100ug) and LC403628 (20ug) can be purchased separately from OriGene.

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  Anti-BTLA mouse monoclonal antibody (TA505536) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTLA (RC219458).

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  Western blot analysis of extracts (35ug) from PBMCs by using anti-BTLA monoclonal antibody (1:500).

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  HEK293T cells transfected with either RC219458 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTLA antibody (TA505536), and then analyzed by flow cytometry (1:500).

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Specifications

Product Data

• Clone Name: OTI4D6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BTLA(NP_861445) produced in HEK293T cell.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.6 kDa
• Gene Name: B and T lymphocyte associated
• Database Link:
  NP_861445
  Entrez Gene 151888 Human
  UniProt ID Q7Z6A9
• Background: This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. [provided by RefSeq, Aug 2011]
• Synonyms: BTLA1; CD272

Reference Data

• Protein Families: Transmembrane