NIPSNAP2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1B8]

CAT#: TA505590BM

GBAS mouse monoclonal antibody,clone 1B8, HRP conjugated

Product Images

Anti-GBAS mouse monoclonal antibody (TA505590) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBAS (RC205027).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GBAS mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GBAS mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GBAS mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GBAS mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GBAS (Cat# RC205027, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBAS(Cat# TA505590). Positive lysates LY400571 (100ug) and LC400571 (20ug) can be purchased separately from OriGene.

• Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-GBAS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-GBAS monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

• Immunofluorescent staining of HeLa cells using anti-GBAS mouse monoclonal antibody (TA505590) at 1:100

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Specifications

Product Data

• Clone Name: OTI1B8
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000, IHC: 1:150, IF: 1:50-1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GBAS(NP_001474) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.6 kDa
• Gene Name: nipsnap homolog 2
• Database Link:
  NP_001474
  Entrez Gene 14467 MouseEntrez Gene 498174 RatEntrez Gene 479700 DogEntrez Gene 715352 MonkeyEntrez Gene 2631 Human
  UniProt ID O75323
• Background: This gene encodes a member of the NipSnap family of proteins that may be involved in vesicular transport. The encoded protein is localized to mitochondria and plays a role in oxidative phosphorylation. A pseudogene of this gene is located on the long arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011]
• Synonyms: NIPSNAP2