C10orf7 (CDC123) Mouse Monoclonal Antibody [Clone ID: OTI1F8]

CAT#: TA505652

CDC123 mouse monoclonal antibody, clone OTI1F8 (formerly 1F8)

Product Images

Immunofluorescent staining of HeLa cells using anti-CDC123 mouse monoclonal antibody (TA505652).

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  Anti-CDC123 mouse monoclonal antibody (TA505652) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDC123 (RC203311).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CDC123 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505652)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CDC123 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505652)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CDC123 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505652)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CDC123 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505652)

• HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC123 (RC203311, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDC123. Positive lysates LY416883 (100ug) and LC416883 (20ug) can be purchased separately from OriGene.

• Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDC123 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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Specifications

Product Data

• Clone Name: OTI1F8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200~4000, IHC 1:150, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CDC123(NP_006014) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39 kDa
• Gene Name: cell division cycle 123
• Database Link:
  NP_006014
  Entrez Gene 98828 MouseEntrez Gene 116656 RatEntrez Gene 478005 DogEntrez Gene 694239 MonkeyEntrez Gene 8872 Human
  UniProt ID O75794
• Synonyms: C10orf7; D123