SAPK4 (MAPK13) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI12B12]

CAT#: TA505781BM

MAPK13 mouse monoclonal antibody,clone 12B12, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK13 (RC200606, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK13. Positive lysates LY400973 (100ug) and LC400973 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAPK13 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• 

  Anti-MAPK13 mouse monoclonal antibody (TA505781) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK13 (RC200606).

Specifications

Product Data

• Clone Name: OTI12B12
• Applications: IF, WB
• Recommended Dilution: WB 1:200~2000, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MAPK13(NP_002745) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.9 kDa
• Gene Name: mitogen-activated protein kinase 13
• Database Link:
  NP_002745
  Entrez Gene 26415 MouseEntrez Gene 29513 RatEntrez Gene 612821 DogEntrez Gene 719085 MonkeyEntrez Gene 5603 Human
  UniProt ID O15264
• Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and cellular stress. MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase. [provided by RefSeq, Jul 2008]
• Synonyms: MAPK-13; MAPK 13; p38delta; PRKM13; SAPK4

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Epithelial cell signaling in Helicobacter pylori infection, Fc epsilon RI signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway, VEGF signaling pathway