MAPK4 Mouse Monoclonal Antibody [Clone ID: OTI2D7]

CAT#: TA505808S

MAPK4 mouse monoclonal antibody, clone OTI2D7 (formerly 2D7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK4 (RC207414, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK4. Positive lysates LY419131 (100ug) and LC419131 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-MAPK4 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MAPK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505808)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MAPK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505808)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAPK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505808)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505808)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAPK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505808)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAPK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505808)

• Anti-MAPK4 mouse monoclonal antibody (TA505808) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK4 (RC207414).

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Specifications

Product Data

• Clone Name: OTI2D7
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MAPK4(NP_002738) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 65.7 kDa
• Gene Name: mitogen-activated protein kinase 4
• Database Link:
  NP_002738
  Entrez Gene 54268 RatEntrez Gene 225724 MouseEntrez Gene 5596 Human
  UniProt ID P31152
• Synonyms: ERK-4; ERK4; p63-MAPK; p63MAPK; PRKM4

Reference Data

• Protein Families: Druggable Genome, Protein Kinase