CD56 (NCAM1) Mouse Monoclonal Antibody [Clone ID: OTI1G4]

CAT#: TA506208S

NCAM1 (CD56) mouse monoclonal antibody, clone OTI1G4 (formerly 1G4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NCAM1 (Cat# RC207890, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NCAM1 (Cat# TA506208). Positive lysates LY405745 (100ug) and LC405745 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NCAM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NCAM1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  User supplied image. Mehmet Fatih B. for TA506208S on 04/03/2023: I stained sections on paraffined tissues by immunohistochemistry. Works perfectly on canine tissue with 1/200 dilution. sharp and very clear staining IHC-method: ABC-peroxidase, AEC chromogen, Gill's hemalum. Tissue: dog neuroendocrine tumor.

Specifications

Product Data

• Clone Name: OTI1G4
• Applications: IHC, WB
• Recommended Dilution: WB 1:200~4000, IHC 1:150
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 20-718 of human NCAM1 (NP_851996) produced in HEK293T cells.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 94.4 kDa
• Gene Name: neural cell adhesion molecule 1
• Database Link:
  NP_851996
  Entrez Gene 17967 MouseEntrez Gene 24586 RatEntrez Gene 479435 DogEntrez Gene 693789 MonkeyEntrez Gene 4684 Human
  UniProt ID P13591
• Background: This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]
• Synonyms: CD56; MSK39; NCAM

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Prion diseases