Galectin 3 (LGALS3) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1A10]

CAT#: TA506395AM

LGALS3 mouse monoclonal antibody, clone OTI1A10 (formerly 1A10), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3 (RC208785, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3. Positive lysates LY400834 (100ug) and LC400834 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LGALS3 monoclonal antibody at 1:400 dilution (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and LGALS3-Knockout Hela cells (KO, Cat# LC810128) were separated by SDS-PAGE and immunoblotted with anti-LGALS3 monoclonal antibody TA506395. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

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  Immunofluorescent staining of HeLa cells using anti-LGALS3 mouse monoclonal antibody (TA506395).

Specifications

Product Data

• Clone Name: OTI1A10
• Applications: IF, WB
• Recommended Dilution: WB 1:400 - 1:2000, IF 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LGALS3(NP_002297) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26 kDa
• Gene Name: galectin 3
• Database Link:
  NP_002297
  Entrez Gene 16854 MouseEntrez Gene 3958 Human
  UniProt ID P17931
• Background: This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
• Synonyms: CBP35; GAL3; GALBP; GALIG; L31; LGALS2; MAC2

Reference Data

• Protein Families: Secreted Protein