IL1 beta (IL1B) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3E1]

CAT#: TA506443AM

IL1B mouse monoclonal antibody,clone 3E1, Biotinylated

Product Images

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human IL1B plasmid (RC202079, hIL1B), mouse IL1B plasmid (MR226719, mIL1B), rat IL1B plasmid (RR211023, rIL1B) using anti-IL1B antibody TA506443 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IL1B mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded normal breast cancer and normal adjacent using anti-IL1B antibody TA506443 clone OTI3E1 mouse monoclonal antibody. Protocol used HIER TEE pH9.0 (cat# B21-100) and anti-IL1B at 1:100 dilution. Detection was done with Polink1 Broad Mouse and Rabbit C/N D11-18 with DAB Kit. Image 40x magnification. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded breast cancer using anti-IL1B antibody TA506443 clone OTI3E1 mouse monoclonal antibody. HIER TEE pH9.0 (cat# B21-100) and anti-IL1B at 1:100 dilution. Detection was done with Polink1 Broad Mouse and Rabbit C/N D11-18 with DAB Kit. Image 40x magnification. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Figure from citation: Immunohistochemistry of IL1B protein level by using anti-IL1B antibody in synovial tissues from fracture and OA patients, respectively. Dilution: 1:100 View Citation. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Anti-IL1B mouse monoclonal antibody (TA506443) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1B (RC202079).

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Specifications

Product Data

• Clone Name: OTI3E1
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:1000~4000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IL1B(NP_000567) produced in HEK293T cell.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.6 kDa
• Gene Name: interleukin 1 beta
• Database Link:
  NP_000567
  Entrez Gene 3553 Human
  UniProt ID P01584
• Background: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
• Synonyms: IL-1; IL1-BETA; IL1F2

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Alzheimer's disease, Apoptosis, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Graft-versus-host disease, Hematopoietic cell lineage, MAPK signaling pathway, NOD-like receptor signaling pathway, Prion diseases, Toll-like receptor signaling pathway, Type I diabetes mellitus