ACTH (POMC) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2B2]

CAT#: TA506610AM

POMC mouse monoclonal antibody, clone OTI2B2 (formerly 2B2), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POMC (RC215351, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POMC. Positive lysates LY422129 (100ug) and LC422129 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-POMC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506610)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-POMC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506610)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POMC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506610)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-POMC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506610)

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Specifications

Product Data

• Clone Name: OTI2B2
• Applications: IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human POMC(NP_001030333) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.6 kDa
• Gene Name: proopiomelanocortin
• Database Link:
  NP_001030333
  Entrez Gene 5443 Human
  UniProt ID P01189
• Synonyms: ACTH; CLIP; LPH; MSH; NPP; POC

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Melanogenesis