GRF2 (RAPGEF1) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2C3]

CAT#: TA506838AM

RAPGEF1 mouse monoclonal antibody,clone 2C3, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAPGEF1 (RC208092, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAPGEF1. Positive lysates LY404802 (100ug) and LC404802 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RAPGEF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RAPGEF1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RAPGEF1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RAPGEF1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RAPGEF1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RAPGEF1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-RAPGEF1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RAPGEF1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RAPGEF1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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Specifications

Product Data

• Clone Name: OTI2C3
• Applications: IHC, WB
• Recommended Dilution: WB 1:400~4000, IHC 1:150
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RAPGEF1(NP_941372) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 122.6 kDa
• Gene Name: Rap guanine nucleotide exchange factor 1
• Database Link:
  NP_941372
  Entrez Gene 107746 MouseEntrez Gene 63881 RatEntrez Gene 491286 DogEntrez Gene 715272 MonkeyEntrez Gene 2889 Human
  UniProt ID Q13905
• Synonyms: C3G; GRF2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Insulin signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma