ICAM1 Mouse Monoclonal Antibody [Clone ID: OTI2H4]

CAT#: TA506870S

ICAM1 mouse monoclonal antibody, clone OTI2H4 (formerly 2H4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM1 (RC200714, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM1 (1:500).

• 

  Western blot analysis of extracts (35ug) from 2 different cell lines and human spleen tissue by using anti-ICAM1 monoclonal antibody (1:100).

• 

  Western blot analysis of extracts (35ug) from A549 cells (-), HUVEC cells (-) and A549 cells treated with 20ng/ml TNF-a for 24h (+), using anti-ICAM1 monoclonal antibody (1:100).

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ICAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506870) (1:150)

• 

  Immunofluorescent staining of living A549 cells treated with 20ng/ml TNF-a for 24h using anti-ICAM1 mouse monoclonal antibody (TA506870, green, upper left; merged, upper right) or untreated A549 cells ( merged, lower right). Cell nuclei were stained with DAPI (blue, lower left) (1:100).

• 

  Immunofluorescent staining of living HUVEC cells treated with 20ng/ml TNF-a for 5h using anti-ICAM1 mouse monoclonal antibody (TA506870, green, upper left; merged, upper right) or untreated HUVEC cells ( merged, lower right). Cell nuclei were stained with DAPI (blue, lower left) (1:100).

• HEK293T cells transfected with either RC200714 overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-ICAM1 antibody (TA506870), and then analyzed by flow cytometry (1:100).

• Flow cytometric Analysis of living HUVEC cells, using anti-ICAM1 antibody (TA506870), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

Show all 8 images

Specifications

Product Data

• Clone Name: OTI2H4
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ICAM1(NP_000192) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55.2 kDa
• Gene Name: intercellular adhesion molecule 1
• Database Link:
  NP_000192
  Entrez Gene 3383 Human
  UniProt ID P05362
• Background: This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
• Synonyms: BB2; CD54; P3.58

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Viral myocarditis