Hydroxysteroid (17 beta) Dehydrogenase 4 (HSD17B4) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI4C4]

CAT#: TA507028AM

HSD17B4 mouse monoclonal antibody,clone 4C4, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HSD17B4 (Cat# RC200460, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD17B4(Cat# TA507028). Positive lysates LY424737 (100ug) and LC424737 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HSD17B4 mouse monoclonal antibody (TA507028) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSD17B4 mouse monoclonal antibody (TA507028) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HSD17B4 mouse monoclonal antibody (TA507028) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-HSD17B4 mouse monoclonal antibody (TA507028) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HSD17B4 mouse monoclonal antibody (TA507028) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HSD17B4 mouse monoclonal antibody (TA507028) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HSD17B4 mouse monoclonal antibody (TA507028) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Anti-HSD17B4 mouse monoclonal antibody (TA507028) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSD17B4 (RC200460).

• Immunofluorescent staining of HeLa cells using anti-HSD17B4 mouse monoclonal antibody (TA507028) at 1:100 dilution.

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Specifications

Product Data

• Clone Name: OTI4C4
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IF 1:100, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HSD17B4(NP_000405) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: hydroxysteroid 17-beta dehydrogenase 4
• Database Link:
  NP_000405
  Entrez Gene 15488 MouseEntrez Gene 3295 Human
  UniProt ID P51659
• Background: The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. [provided by RefSeq, Jul 2008]
• Synonyms: DBP; MFE-2; MPF-2; PRLTS1; SDR8C1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Primary bile acid biosynthesis