PON1 Rabbit Polyclonal Antibody

CAT#: TA590020

Rabbit Polyclonal PON1 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (RC210356, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PON1. Positive lysates LY400156 (100ug) and LC400156 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PON1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  ELISA: PON1 Antibody

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PON1 rabbit polyclonal antibody. (TA590020)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PON1 rabbit polyclonal antibody. (TA590020)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PON1 rabbit polyclonal antibody. (TA590020)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PON1 rabbit polyclonal antibody. (TA590020)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PON1 rabbit polyclonal antibody. (TA590020)

• Immunofluorescent staining of HeLa cells using anti-PON1 rabbit polyclonal antibody (TA590020).

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Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: WB 1:5000~20000, IHC 1:150, IF 1:500,ELISA 1:100-1:2000
• Reactivities: Human, Rat, Dog
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody is specific for the N Terminus Region of the target protein.
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: paraoxonase 1
• Database Link:
  NP_000437
  Entrez Gene 84024 RatEntrez Gene 475234 DogEntrez Gene 5444 Human
  UniProt ID P27169
• Background: The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq]
• Synonyms: ESA; MVCD5; PON
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Metabolic pathways