IGF2BP2 Rabbit Polyclonal Antibody

CAT#: TA590227

Rabbit Polyclonal IGF2BP2 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (RC205673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2. Positive lysates LY401961 (100ug) and LC401961 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Western Blot: IGF2BP2 Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil Target weight: 66, 62 (splice variants) Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

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  ELISA: IGF2BP2 Antibody

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  Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.Antibody dilution:1:400. Image and statement courtesy of the Human Protein Atlas (HPA).

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  Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm. Antibody dilution: 1:100. Image and statement courtesy of the Human Protein Atlas (HPA).

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Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: WB 1:5000~20000, ELISA 1:100-1:2000
• Reactivities: Human, Monkey, Mouse, Dog
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody is specific for the N Terminus Region of the target protein.
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 0.9mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: insulin like growth factor 2 mRNA binding protein 2
• Database Link:
  NP_006539
  Entrez Gene 319765 MouseEntrez Gene 478662 DogEntrez Gene 700598 MonkeyEntrez Gene 10644 Human
  UniProt ID Q9Y6M1
• Background: This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
• Synonyms: IMP-2; IMP2; VICKZ2
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT