Cyclin B1 (CCNB1) Rabbit Polyclonal Antibody

CAT#: TA590439

Rabbit Polyclonal Cyclin B1 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNB1 (RC200812, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNB1.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CCNB1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  ELISA: Cyclin B1 Antibody

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CCNB1 rabbit polyclonal antibody. (TA590439)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CCNB1 rabbit polyclonal antibody. (TA590439)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CCNB1 rabbit polyclonal antibody. (TA590439)

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Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody is specific for the N Terminus Region of the target protein.
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 0.81168 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cyclin B1
• Database Link:
  NP_114172
  Entrez Gene 25203 RatEntrez Gene 268697 MouseEntrez Gene 608420 DogEntrez Gene 699504 MonkeyEntrez Gene 891 Human
  UniProt ID P14635
• Background: In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is Ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
• Synonyms: CCNB
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation