NAD Synthetase (NADSYN1) Rabbit Polyclonal Antibody

CAT#: TA590494

Rabbit Polyclonal NAD Synthetase Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NADSYN1 (RC200131, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NADS. Positive lysates LY413259 (100ug) and LC413259 (20ug) can be purchased separately from OriGene.

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  ELISA: NAD Synthetase Antibody

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  Immunohistochemistry: NAD Synthetase Antibody - Most of the normal tissues displayed weak to moderate cytoplasmic positivity. Neuronal cells in the CNS showed moderate nuclear membrane staining. Squamous epithelia, breast and prostate glands were mainly negative. A few cases of gliomas, melanomas and urothelial cancers exhibited weak to moderate cytoplasmic immunoreactivity. The remaining tumors were basically negative. Image and statement courtesy of the Human Protein Atlas (HPA).

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  Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in proximal tubules.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

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  Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

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Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: WB 1:5000~20000, ELISA 1:100-1:2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody is specific for the Middle Region of the target protein.
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 0.75mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: NAD synthetase 1
• Database Link:
  NP_060631
  Entrez Gene 55191 Human
  UniProt ID Q6IA69
• Background: Nicotinamide adenine dinucleotide (NAD) is a coenzyme in metabolic redox reactions, a precursor for several cell signaling molecules, and a substrate for protein posttranslational modifications. NAD synthetase (EC 6.3.5.1) catalyzes the final step in the biosynthesis of NAD from nicotinic acid adenine dinucleotide (NaAD). [supplied by OMIM]
• Synonyms: FLJ10631; FLJ36703; FLJ40627
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism