BOB1 (POU2AF1) Rabbit Polyclonal Antibody

CAT#: TA590578

Rabbit Polyclonal BOB1 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2. Positive lysates LY416779 (100ug) and LC416779 (20ug) can be purchased separately from OriGene.

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  ELISA: BOB1 Antibody

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: WB 1:5000~20000,ELISA 1:100-1:2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody is specific for the C Terminus Region of the target protein.
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: POU class 2 homeobox associating factor 1
• Database Link:
  NP_006226
  Entrez Gene 5450 Human
  UniProt ID Q16633
• Background: A novel 35kDa protein designated Bob1 (B cell Oct binding protein 1) is a lymphocyte specific transcription co activator protein. B cell specific transcription is mediated by octamer motifs found in immunoglobulin heavy and light chain gene promoters and in some immunoglobulin enhancers. These sites are bound by the B cell specific Oct1 and Oct2 transcription factors, and the B cell restricted co activator BOB.1/OBF.1. This protein interacts only with the Oct1/2 proteins through sub domains in the POU domain of the Oct1/2 proteins, enhancing their transcriptional efficacy. Although having no intrinsic capacity for DNA binding, Bob1 associates tightly with the octomer motif in the presence of Oct1 and Oct2. Bob1 is expressed at highest levels in spleen and peripheral blood leukocytes.
• Synonyms: BOB1; OBF-1; OBF1; OCAB
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT