JUND Rabbit Polyclonal Antibody

CAT#: TA590607

Rabbit Polyclonal JunD Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JUND (RC216958, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JUND. Positive lysates LY417361 (100ug) and LC417361 (20ug) can be purchased separately from OriGene.

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  ELISA: JunD Antibody

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-JUND Rabbit Polyclonal antibody. (TA590607)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-JUND Rabbit Polyclonal antibody. (TA590607)

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody is specific for the N Terminus Region of the target protein.
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 0.74mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: JunD proto-oncogene, AP-1 transcription factor subunit
• Database Link:
  NP_005345
  Entrez Gene 3727 Human
  UniProt ID P17535
• Background: JunD is the most broadly expressed member of the Jun family and the AP1 transcription factor complex. It has been found that primary fibroblasts lacking murine JunD displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, JunD -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JunD was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JunD protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53.
• Synonyms: AP-1
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: MAPK signaling pathway