HDAC2 Rabbit Polyclonal Antibody

CAT#: TA590734

Rabbit Polyclonal HDAC2 Antibody

Product Images

ELISA: HDAC2 Antibody

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-HDAC2 rabbit polyclonal antibody. (TA590734)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-HDAC2 rabbit polyclonal antibody. (TA590734)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HDAC2 rabbit polyclonal antibody. (TA590734)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HDAC2 rabbit polyclonal antibody. (TA590734)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HDAC2 rabbit polyclonal antibody. (TA590734)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HDAC2 rabbit polyclonal antibody. (TA590734)

• HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC2 (RC224919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC2. Positive lysates LY419878 (100ug) and LC419878 (20ug) can be purchased separately from OriGene.

• Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC2 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody is specific for the C Terminus Region of the target protein.
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 0.66934 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: histone deacetylase 2
• Database Link:
  NP_001518
  Entrez Gene 15182 MouseEntrez Gene 84577 RatEntrez Gene 475035 DogEntrez Gene 694662 MonkeyEntrez Gene 3066 Human
  UniProt ID Q92769
• Background: Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.
• Synonyms: HD2; RPD3; YAF1
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, Huntington's disease, Notch signaling pathway, Pathways in cancer