SLUG (SNAI2) Mouse Monoclonal Antibody [Clone ID: OTI1A6]

CAT#: TA800167S

SNAI2 (SLUG) mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)

Product Images

HEK293T cells transfected with either RC202365 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAI2 antibody (TA800167), and then analyzed by flow cytometry.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SNAI2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SNAI2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SNAI2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SNAI2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNAI2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SNAI2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human SNAI2 plasmid (RC202365, lane 2), mouse SNAI2 plasmid (MR203571, lane 3),rat SNAI2 plasmid (RR213332, lane 4), using anti-SNAI2 antibody TA800167 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

• Figure from citation: Immunohistochemical analysis of Slug (also known as SNAI2) on serial sections of cartilage from normal and OA cartilage by using anti-SNAI2 antibody. Bar=100 um, Inset bar=10 um. Dilution: 1:300 View Citation. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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Specifications

Product Data

• Clone Name: OTI1A6
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Rat, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SNAI2 (NP_003059) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.8 kDa
• Gene Name: snail family transcriptional repressor 2
• Database Link:
  NP_003059
  Entrez Gene 20583 MouseEntrez Gene 25554 RatEntrez Gene 6591 Human
  UniProt ID O43623
• Background: This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq]
• Synonyms: SLUG; SLUGH1; SNAIL2; WS2D

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Adherens junction