RIP (RIPK1) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2D6]

CAT#: TA800312BM

RIPK1 mouse monoclonal antibody,clone 2D6, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RIPK1 (Cat# RC216024, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIPK1(Cat# TA800312). Positive lysates LY401251 (100ug) and LC401251 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-RIPK1 monoclonal antibody at 1:200 dilution.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RIPK1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RIPK1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RIPK1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RIPK1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RIPK1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RIPK1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RIPK1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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Specifications

Product Data

• Clone Name: OTI2D6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 133-422 of human RIPK1(NP_003795) produced in E.coli.
• Formulation: PBS (pH 7.4) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 75.8 kDa
• Gene Name: receptor interacting serine/threonine kinase 1
• Database Link:
  NP_003795
  Entrez Gene 8737 Human
  UniProt ID Q13546
• Synonyms: RIP; RIP-1; RIP1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway