PHKG2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2A3]

CAT#: TA800354AM

PHKG2 mouse monoclonal antibody,clone 2A3, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHKG2 (RC200597, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHKG2. Positive lysates LY400111 (100ug) and LC400111 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HEK293T cells (WT, Cat# LC810293T) and PHKG2-Knockout HEK293T cells (KO, Cat# LC840780) were separated by SDS-PAGE and immunoblotted with anti-PHKG2 monoclonal antibody TA800354 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Anti-PHKG2 mouse monoclonal antibody (TA800354) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PHKG2 (RC200597).

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  HEK293T cells transfected with either RC200597 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PHKG2 antibody (TA800354), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2A3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-216 of human PHKG2 (NP_000285) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.3 kDa
• Gene Name: phosphorylase kinase catalytic subunit gamma 2
• Database Link:
  NP_000285
  Entrez Gene 68961 MouseEntrez Gene 5261 Human
  UniProt ID P15735
• Background: Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
• Synonyms: GSD9C

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, Insulin signaling pathway