WDR74 Mouse Monoclonal Antibody [Clone ID: OTI4B2]

CAT#: TA800467

WDR74 mouse monoclonal antibody, clone OTI4B2 (formerly 4B2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY WDR74 (Cat# RC221134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR74(Cat# TA800467). Positive lysates LY413318 (100ug) and LC413318 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-WDR74 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-WDR74 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-WDR74 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Specifications

Product Data

• Clone Name: OTI4B2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 114-385 of human WDR74 (NP_060563) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.3 kDa
• Gene Name: WD repeat domain 74
• Database Link:
  NP_060563
  Entrez Gene 107071 MouseEntrez Gene 690229 RatEntrez Gene 483778 DogEntrez Gene 718460 MonkeyEntrez Gene 54663 Human
  UniProt ID Q6RFH5
• Synonyms: FLJ10439; FLJ21730