SERBP1 Mouse Monoclonal Antibody [Clone ID: OTI4G2]

CAT#: TA800686

SERBP1 mouse monoclonal antibody, clone OTI4G2 (formerly 4G2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERBP1 (RC204527, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERBP1. Positive lysates LY422719 (100ug) and LC422719 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERBP1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and SERBP1-Knockout 293T cells (KO, Cat# LC811318) were separated by SDS-PAGE and immunoblotted with anti-SERBP1 monoclonal antibody TA800686, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SERBP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SERBP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SERBP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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Specifications

Product Data

• Clone Name: OTI4G2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-262 of human SERBP1 (NP_001018078) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.1 kDa
• Gene Name: SERPINE1 mRNA binding protein 1
• Database Link:
  NP_001018078
  Entrez Gene 66870 MouseEntrez Gene 246303 RatEntrez Gene 479535 DogEntrez Gene 700797 MonkeyEntrez Gene 26135 Human
  UniProt ID Q8NC51
• Synonyms: CGI-55; CHD3IP; HABP4L; PAI-RBP1; PAIRBP1