Cytokeratin 5 (KRT5) Mouse Monoclonal Antibody [Clone ID: OTI1C7]

CAT#: TA800728

KRT5 (Keratin 5) mouse monoclonal antibody, clone OTI1C7 (formerly 1C7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT5 (RC206870, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT5. Positive lysates LY400149 (100ug) and LC400149 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-KRT5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA800728) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800728)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800728)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800728)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (TA800728) (1:1000)

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Specifications

Product Data

• Clone Name: OTI1C7
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide: EQGTKTVRQN LEPLFEQYIN NLRRQ, corresponding to the conserved region of human KRT5 and KRT6
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 62.2 kDa
• Gene Name: keratin 5
• Database Link:
  NP_000415
  Entrez Gene 110308 MouseEntrez Gene 369017 RatEntrez Gene 3852 Human
  UniProt ID P13647
• Background: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
• Synonyms: CK5; DDD; DDD1; EBS2; K5; KRT5A