MGMT Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1B12]

CAT#: TA800732BM

MGMT mouse monoclonal antibody,clone 1B12, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT (RC229131, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGMT. Positive lysates LY432155 (100ug) and LC432155 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-MGMT monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800732)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800732)

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800732)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MGMT mouse monoclonal antibody. (TA800732) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (TA800732) Dilution: 1:150

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Specifications

Product Data

• Clone Name: OTI1B12
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MGMT (NP_002403) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: O-6-methylguanine-DNA methyltransferase
• Database Link:
  NP_002403
  Entrez Gene 4255 Human
  UniProt ID P16455
• Synonyms: methylguanine-DNA methyltransferase; O-6-methylguanine-DNA methyltransferase; O6-methylguanine-DNA methyltransferase; OTTHUMP00000020741

Reference Data

• Protein Families: Druggable Genome