S100A1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI6G4]

CAT#: TA800811AM

S100A1 mouse monoclonal antibody,clone 6G4, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100A1 (RC200278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A1. Positive lysates LY401900 (100ug) and LC401900 (20ug) can be purchased separately from OriGene.

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  Standard curve for ELISA analysis with S100A1 recombinant protein (dilution range from 100pg/ml to 1ug/ml) using S100A1 Capture Antibody (Cat# TA801079) at 5ug/ml and HRP conjugated S100A1 Detection mAb (Cat# TA800811) at 0.033ug/ml.

Specifications

Product Data

• Clone Name: OTI6G4
• Applications: ELISA, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human S100A1 (NP_006262) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 10.4 kDa
• Gene Name: S100 calcium binding protein A1
• Database Link:
  NP_006262
  Entrez Gene 20193 MouseEntrez Gene 295214 RatEntrez Gene 6271 Human
  UniProt ID P23297
• Background: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq, Jul 2008]
• Synonyms: S100; S100-alpha; S100A