P Glycoprotein (ABCB1) Mouse Monoclonal Antibody [Clone ID: OTI2C7]

CAT#: TA801006

ABCB1 mouse monoclonal antibody, clone OTI2C7 (formerly 2C7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or fragment (995-1280 AA) of ABCB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCB1.

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  Western blot analysis of extracts (15ug) from 4 different cell lines by using anti-ABCB1 monoclonal antibody (Lane 1: 293T; Lane 2: UO_31; Lane 3: HCT_115; Lane 4: Hela)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801006)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801006)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801006)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801006)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801006)

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Specifications

Product Data

• Clone Name: OTI2C7
• Applications: IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 995-1280 of human ABCB1 (NP_000918) produced in SF9 cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ATP binding cassette subfamily B member 1
• Database Link:
  NP_000918
  Entrez Gene 170913 RatEntrez Gene 5243 Human
  UniProt ID P08183
• Background: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq, Jul 2008]
• Synonyms: ABC20; CD243; CLCS; GP170; MDR1; P-GP; PGY1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: ABC transporters