HIF 2 alpha (EPAS1) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2A6]

CAT#: TA801749AM

EPAS1 mouse monoclonal antibody, clone OTI2A6 (formerly 2A6), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPAS1 (RC208604, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPAS1 (1:2000). Positive lysates LY400555 (100ug) and LC400555 (20ug) can be purchased separately from OriGene.

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  HEK293T cells were transfected with the pCMV6-HIF1A (RC202461), pCMV6-EPAS1 (RC208604) and pCMV6-HIF3A (RC210255) (1:2000).

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-EPAS1 mouse monoclonal antibody. (TA801749)

Specifications

Product Data

• Clone Name: OTI2A6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 584-870 of human EPAS1 (NP_001421) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 96.3 kDa
• Gene Name: endothelial PAS domain protein 1
• Database Link:
  NP_001421
  Entrez Gene 13819 MouseEntrez Gene 29452 RatEntrez Gene 2034 Human
  UniProt ID Q99814
• Background: This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]
• Synonyms: bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma