Thymidylate Synthase (TYMS) Mouse Monoclonal Antibody [Clone ID: OTI1B2]

CAT#: TA801762S

TYMS mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYMS (RC204814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYMS. Positive lysates LY420700 (100ug) and LC420700 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-TYMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA801762) (1:500)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TYMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, TA801762) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TYMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA801762) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TYMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA801762) (1:500)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TYMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA801762) (1:500)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TYMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801762)

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Specifications

Product Data

• Clone Name: OTI1B2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TYMS (NP_001062) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: thymidylate synthetase
• Database Link:
  NP_001062
  Entrez Gene 22171 MouseEntrez Gene 29261 RatEntrez Gene 7298 Human
  UniProt ID P04818
• Background: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008]
• Synonyms: HST422; TMS; TS

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, One carbon pool by folate, Pyrimidine metabolism