Hamartin (TSC1) Mouse Monoclonal Antibody [Clone ID: OTI5A8]

CAT#: TA801951

TSC1 mouse monoclonal antibody, clone OTI5A8 (formerly 5A8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1 (RC213332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC1. Positive lysates LY400132 (100ug) and LC400132 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801951)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801951)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801951)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-TSC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801951)

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Specifications

Product Data

• Clone Name: OTI5A8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 836-1164 of human TSC1 (NP_000359) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 129.6 kDa
• Gene Name: tuberous sclerosis 1
• Database Link:
  NP_000359
  Entrez Gene 60445 RatEntrez Gene 64930 MouseEntrez Gene 7248 Human
  UniProt ID Q92574
• Background: This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun
• Synonyms: LAM; TSC

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway