AREB6 (ZEB1) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3G6]

CAT#: TA802298AM

ZEB1 mouse monoclonal antibody,clone 3G6, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1. Positive lysates LY403073 (100ug) and LC403073 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ZEB1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

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  Cross-reactivity test with ZEB2: HEK293T cells were transfected with plasmids overexpressing either ZEB2 (left) or ZEB1 (right) and immunoblotted with anti-ZEB1 (TA802298).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802298)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802298)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802298)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802298)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802298)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802298)

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Specifications

Product Data

• Clone Name: OTI3G6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-350 of human ZEB1 (NP_110378) produced in E.coli.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 123.9 kDa
• Gene Name: zinc finger E-box binding homeobox 1
• Database Link:
  NP_110378
  Entrez Gene 21417 MouseEntrez Gene 25705 RatEntrez Gene 6935 Human
  UniProt ID P37275
• Background: This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]
• Synonyms: AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3; TCF8; ZFHEP; ZFHX1A
• Note: This ZEB1 antibody (TA802298) does not cross-react with ZEB2

Reference Data

• Protein Families: Transcription Factors