NDUFS2 Mouse Monoclonal Antibody [Clone ID: OTI1D5]

CAT#: TA802340

NDUFS2 mouse monoclonal antibody,clone OTI1D5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFS2 (RC203485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFS2. Positive lysates LY417919 (100ug) and LC417919 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of A549 cell lysate (35ug) by using anti-NDUFS2 monoclonal antibody. Dilution: 1:500

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802340)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802340)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802340)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802340)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802340)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802340)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802340)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802340)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802340)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802340)

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Specifications

Product Data

• Clone Name: OTI1D5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 205-463 of human NDUFS2 (NP_004541) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.1 kDa
• Gene Name: NADH:ubiquinone oxidoreductase core subunit S2
• Database Link:
  NP_004541
  Entrez Gene 226646 MouseEntrez Gene 289218 RatEntrez Gene 4720 Human
  UniProt ID O75306
• Background: The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
• Synonyms: CI-49

Reference Data

• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease