PARN Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4E12]

CAT#: TA802650BM

PARN mouse monoclonal antibody,clone 4E12, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARN (RC207220, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARN. Positive lysates LY419233 (100ug) and LC419233 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of HT29 cell lysate (35ug) by using anti-PARN monoclonal antibody. Dilution: 1:500

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PARN mouse monoclonal antibody. (TA802650) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PARN mouse monoclonal antibody. (TA802650) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PARN mouse monoclonal antibody. (TA802650) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PARN mouse monoclonal antibody. (TA802650) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PARN mouse monoclonal antibody. (TA802650) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PARN mouse monoclonal antibody. (TA802650) Dilution: 1:150

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Specifications

Product Data

• Clone Name: OTI4E12
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-266 of human PARN (NP_002573) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.3 kDa
• Gene Name: poly(A)-specific ribonuclease
• Database Link:
  NP_002573
  Entrez Gene 74108 MouseEntrez Gene 5073 Human
  UniProt ID O95453
• Background: The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
• Synonyms: DAN

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation