Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody [Clone ID: OTI10D5]

CAT#: TA802663S

KRT20 mouse monoclonal antibody, clone OTI10D5 (formerly 10D5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT20 (RC206784, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT20. Positive lysates LY402725 (100ug) and LC402725 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KRT20 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA802663) (1:600)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KRT20 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA802663) (1:600)

Specifications

Product Data

• Clone Name: OTI10D5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human KRT20 (NP_061883) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48.3 kDa
• Gene Name: keratin 20
• Database Link:
  NP_061883
  Entrez Gene 54474 Human
  UniProt ID P35900
• Background: The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
• Synonyms: CD20; CK-20; CK20; K20; KRT21