TIF1 alpha (TRIM24) Mouse Monoclonal Antibody [Clone ID: OTI2D9]

CAT#: TA802797S

TRIM24 mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM24 (RC218319, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM24.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and TRIM24-Knockout Hela cells (KO, Cat# [LC810278]) were separated by SDS-PAGE and immunoblotted with anti-TRIM24 monoclonal antibody TA802797, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TRIM24 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802797)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TRIM24 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802797)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TRIM24 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802797)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TRIM24 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802797)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TRIM24 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802797)

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Specifications

Product Data

• Clone Name: OTI2D9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 706-1016 of human TRIM24 (NP_003843) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tripartite motif containing 24
• Database Link:
  NP_003843
  Entrez Gene 21848 MouseEntrez Gene 8805 Human
  UniProt ID O15164
• Background: The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A; TIF1ALPHA

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors