FBXW7 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI10B9]

CAT#: TA802871AM

FBXW7 mouse monoclonal antibody, clone OTI10B9 (formerly 10B9), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXW7 (RC217398, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXW7. Positive lysates LY403254 (100ug) and LC403254 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-FBXW7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA802871)

Specifications

Product Data

• Clone Name: OTI10B9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-354 of human FBXW7 (NP_361014) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 79.5 kDa
• Gene Name: F-box and WD repeat domain containing 7
• Database Link:
  NP_361014
  Entrez Gene 55294 Human
  UniProt ID Q969H0
• Synonyms: AGO; CDC4; FBW6; FBW7; FBX30; FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Ubiquitin mediated proteolysis