Cytokeratin 7 (KRT7) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1B3]

CAT#: TA803026BM

KRT7 mouse monoclonal antibody,clone 1B3, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT7 (RC201124, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT7.

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  Western blot analysis of A549 cell lysate (35ug) by using anti-KRT7 monoclonal antibody. Dilution: 1:500

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-KRT7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803026)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-KRT7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803026)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-KRT7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803026)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-KRT7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803026)

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Specifications

Product Data

• Clone Name: OTI1B3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-114 and 340-469 of human KRT7 (NP_005547) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.2 kDa
• Gene Name: keratin 7
• Database Link:
  NP_005547
  Entrez Gene 3855 Human
  UniProt ID P08729
• Background: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
• Synonyms: CK7; K2C7; K7; SCL

Reference Data

• Protein Families: ES Cell Differentiation/IPS