VEGFA Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2E11]

CAT#: TA803421AM

VEGFA mouse monoclonal antibody,clone OTI2E11, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGFA (RC223789, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGFA (1:500).

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  HEK293T cells were transfected with the overexpression plasmids of 9 VEGF isoforms (from left to right:VEGF206, RC223789; VEGF189, RC229706; L-VEGF165, RC223884; VEGF165, RC229662; L-VEGF121, RC222129; VEGF iso18, RC229874; VEGF183, RC229686; VEGF145, RC231952; L-VEGF189, RC224244) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-flag antibody (TA180144, 1:1000) or anti-VEGFA mouse monoclonal antibody. (TA803421, 1:500)

Specifications

Product Data

• Clone Name: OTI2E11
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 206-412 of human VEGFA (NP_001020537) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.3 kDa
• Gene Name: vascular endothelial growth factor A
• Database Link:
  NP_001020537
  Entrez Gene 7422 Human
  UniProt ID P15692
• Synonyms: MVCD1; VEGF; VPF

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Bladder cancer, Cytokine-cytokine receptor interaction, Focal adhesion, mTOR signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, VEGF signaling pathway