CEACAM5 Mouse Monoclonal Antibody [Clone ID: OTI3D8]

CAT#: TA803456

CEA (CEACAM5) mouse monoclonal antibody, clone OTI3D8 (formerly 3D8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CEACAM5 (Cat# RC206434, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CEACAM5(Cat# TA803456).

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  Human colon cancer screened with OriGene's Ms anti CEA (TA803456 @ 1:400). HIER buffer TEE (B21-100) was used in pressure cooker. Secondary Polink 1 AP Mouse with out chromogen (D18-110) was use according to protocol data sheet. Cytoplasmic and membrane stain was visualized with GBI permanent red chromogen (C13-120).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CEACAM5 mouse monoclonal antibody. (TA803456; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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Specifications

Product Data

• Clone Name: OTI3D8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 35-680 of human CEACAM5 (NP_004354) produced in SF9 cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: carcinoembryonic antigen related cell adhesion molecule 5
• Database Link:
  NP_004354
  Entrez Gene 1048 Human
  UniProt ID P06731
• Background: Members of the CEACAM subfamily, including CEACAM5, belong to the CEA gene family. For general information on the CEA gene family, see CEACAM1 (MIM 109770). [supplied by OMIM, Oct 2009]
• Synonyms: CD66e; CEA