A RAF (ARAF) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2G4]

CAT#: TA803662AM

ARAF mouse monoclonal antibody, clone OTI2G4 (formerly 2G4), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARAF (RC200737, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARAF. Positive lysates LY400623 (100ug) and LC400623 (20ug) can be purchased separately from OriGene.

• 

  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and ARAF-Knockout 293T cells (KO, Cat# LC810746) were separated by SDS-PAGE and immunoblotted with anti-ARAF monoclonal antibody TA803662 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

Specifications

Product Data

• Clone Name: OTI2G4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 145-365 of human ARAF (NP_001645) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 67.4 kDa
• Gene Name: A-Raf proto-oncogene, serine/threonine kinase
• Database Link:
  NP_001645
  Entrez Gene 11836 MouseEntrez Gene 64363 RatEntrez Gene 369 Human
  UniProt ID P10398
• Synonyms: A-RAF; ARAF1; PKS2; RAFA1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, Melanoma, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Vascular smooth muscle contraction