ErbB 3 (ERBB3) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3E10]

CAT#: TA803694BM

ERBB3 mouse monoclonal antibody, clone OTI3E10 (formerly 3E10), HRP conjugated

Product Images

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ERBB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803694)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ERBB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803694)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ERBB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803694)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ERBB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803694)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ERBB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803694)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ERBB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803694)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-ERBB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803694)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ERBB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803694)

• HEK293T cells transfected with either RC209954 overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-ERBB3 antibody (TA803694), and then analyzed by flow cytometry (1:100).

• Flow cytometric Analysis of living MDA-MB-435 cells, using anti-ERBB3 antibody (TA803694), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:25).

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Specifications

Product Data

• Clone Name: OTI3E10
• Applications: FC
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ERBB3 (NP_001973) produced in SF9 cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 146 kDa
• Gene Name: erb-b2 receptor tyrosine kinase 3
• Database Link:
  NP_001973
  Entrez Gene 13867 MouseEntrez Gene 29496 RatEntrez Gene 2065 Human
  UniProt ID P21860
• Background: This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]
• Synonyms: c-erbB-3; c-erbB3; ErbB-3; erbB3-S; HER3; LCCS2; MDA-BF-1; p45-sErbB3; p85-sErbB3; p180-ErbB3

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Protein Kinase, Secreted Protein, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Calcium signaling pathway, Endocytosis, ErbB signaling pathway