Cytokeratin 19 (KRT19) Mouse Monoclonal Antibody [Clone ID: OTI5H6]

CAT#: TA804075S

KRT19 mouse monoclonal antibody, clone OTI5H6 (formerly 5H6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT19 (RC209707, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT19. Positive lysates LY419428 (100ug) and LC419428 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 cell lines by using anti-KRT19 monoclonal antibody at 1:200.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KRT19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804075)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804075)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KRT19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804075)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KRT19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804075)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-KRT19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804075)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804075)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-KRT19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804075)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-KRT19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804075)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KRT19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804075)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-KRT19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804075)

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Specifications

Product Data

• Clone Name: OTI5H6
• Applications: IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 240-390 of human KRT19 (NP_002267) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.9 kDa
• Gene Name: keratin 19
• Database Link:
  NP_002267
  Entrez Gene 3880 Human
  UniProt ID P08727
• Background: The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
• Synonyms: CK19; K1CS; K19