FAF1 Mouse Monoclonal Antibody [Clone ID: OTI2F9]

CAT#: TA804160S

FAF1 mouse monoclonal antibody, clone OTI2F9 (formerly 2F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAF1 (RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1. Positive lysates LY416234 (100ug) and LC416234 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of HT29 cell lysate (35ug) by using anti-FAF1 monoclonal antibody. Dilution: 1:500

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FAF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804160)

Specifications

Product Data

• Clone Name: OTI2F9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 415-650 of human FAF1 (NP_008982) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.8 kDa
• Gene Name: Fas associated factor 1
• Database Link:
  NP_008982
  Entrez Gene 14084 MouseEntrez Gene 140657 RatEntrez Gene 11124 Human
  UniProt ID Q9UNN5
• Background: Interaction of Fas ligand (TNFSF6) with the FAS antigen (TNFRSF6) mediates programmed cell death, also called apoptosis, in a number of organ systems. The protein encoded by this gene binds to FAS antigen and can initiate apoptosis or enhance apoptosis initiated through FAS antigen. Initiation of apoptosis by the protein encoded by this gene requires a ubiquitin-like domain but not the FAS-binding domain. [provided by RefSeq, Jul 2008]
• Synonyms: CGI-03; hFAF1; HFAF1s; UBXD12; UBXN3A

Reference Data

• Protein Families: Druggable Genome